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The sections were then stained with Periodic Acid Schiff [Sigma-Aldrich] to evaluate kidney morphology. In the end the slides were rinsed in running faucet water, dehydrated, cleared and mounted in mounting medium PROTOCOL XYLENE BASED . The proliferative exercise and the apoptosis of tubular cells had been in contrast between the experimental groups on the totally different time factors . This is considered one of the most informative blogs which I even have seen related to the safety of the live/ video on demand. It is useful in making an knowledgeable decision about the most useful method to secure your movies. Whether it is securing the copyrights of your videos and using its anti-capture feature, VideoCrypt supplies that all.
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Before in vivo injections, a easy in vitro check was employed to discover out the minimum amount of hAFSC detectable by bioluminescence. Different concentrations of the cells ranging from 5×104 to 2×106 had been evaluated. In addition, the expression of the luciferase gene was confirmed by PCR after 20 passages in tradition.
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Detection of the PCR amplification merchandise was performed by measurement fractionation on 1% agarose gel electrophoresis. As a housekeeping gene, amplification of fragments of the human β-actin RNA was performed. Specific human primer sequences, predicted sizes of amplicons and specific annealing temperatures are shown in Table 1. A. Frozen section of a kidney injected with hAFSC after 1 week. The cells are evident as pink fluorescence of the floor marker CM-DiI. paito california -fluorescent staining of injected kidney with luciferase transduced hAFSC (and not CM-Dil labeled), showing the same cells expressing each Aquaporin 2 and luciferase , three weeks after injection.